In a University of Nebraska copy of the 1852 edition of Uncle Tom’s Cabin, part of the Lowenberg Collection, a reader used a stick pin to re-attach the illustration between pgs. 174 and 175 of volume 1. I would think this pin provides pretty good evidence that it was read by a female reader. The pin has rusted, the illustration is no longer attached, but the pin remains, as do pin marks that hold it together.
The reader, I will say “she,” appears to have lavished considerable care on the volume in other ways. On volume 1, pg. 27, the absent chapter title “The Mother” is restored in pencil, presumably from the table of contents.
In the Typee manuscript, John Bryant notes that Melville used pins to attach revision pages. Many of the revision sheets are since detached, but the pins remain. A history of reader’s pins and pin marks, anyone?